Direct cytoplasmic delivery of protein drugs by electric field-driven cell membrane-permeable peptide nanoneedles

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K12800
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Waku Tomonori 京都工芸繊維大学, 分子化学系, 准教授 (30548699)
• Co-Investigator(Kenkyū-buntansha): 小堀 哲生 京都工芸繊維大学, 分子化学系, 教授 (00397605)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: ドラッグデリバリーシステム / 自己組織化 / ペプチド / ナノ材料 / ナノファイバー

Outline of Final Research Achievements

Aiming at the development of a carrier for cytoplasmic delivery of protein drugs, we have developed a technique for preparing peptide nanofibers with a multi-block structure as an element technology necessary for the preparation. Even with combinations of seeds and monomers with different compositions, if the fibril-forming sequences were common, fibers were elongated from the seeds, and nanofibers with a multi-block structure consisting of heterogeneous segments were formed. It was also found that the length of each segment can be freely controlled.

Free Research Field

高分子化学、バイオマテリアル

Academic Significance and Societal Importance of the Research Achievements

本研究で開発したマルチブロック構造を有するペプチドナノファイバーの作製技術は、従来の高分子ミセルなどの球状キャリアとは異なる機能を有する新たなドラッグデリバリーキャリアの開発に役立つと期待される。