Peptide based biomaterials applied for 3D culture and tissue engineeringgineering

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K12810
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: Kansai University
• Principal Investigator: Hirano Yoshiaki 関西大学, 化学生命工学部, 教授 (80247874)
• Co-Investigator(Kenkyū-buntansha): 大槻 周平 大阪医科薬科大学, 医学部, 講師 (20589840)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 組織工学 / 足場材料 / ペプチドハイドロゲル / 間葉系幹細胞 / 細胞集合体 / 軟骨 / 半月板 / RGDSペプチド

Outline of Final Research Achievements

The purpose of this project is to construct 3D tissues (especially cartilage and meniscus tissues) and organoid-like cell assemblies by combining the cell aggregate inducing technique and hydrogel scaffolds that the applicant has revealed. As for the cell aggregate inducing peptides, it was also found that they do not exert any negative factors on cells. Functional analysis of cell aggregation encapsulated peptide hydrogels revealed that cell function is improved by embedding the peptides in gels with cell assemblies.In addition, evaluation of meniscus regeneration using the cell aggregation encapsulated peptide hydrogels showed remarkable tissue regeneration.
These peptides were also effective in treating the area around the joint.

Free Research Field

生体材料科学・ペプチド工学

Academic Significance and Societal Importance of the Research Achievements

細胞集合体誘導ペプチドと自己組織化ペプチドハイドロゲルを組み合わせることにより、効率よく組織再生や細胞機能を向上させることが明らかになった。特に、分子設計したペプチドハイドロゲルは、関節部位の組織工学用足場材料として有用であることが明らかになった。
以上より、組織工学的手法が整形外科分野の関節領域に有効であることが分かり、さらなる医療分野への展開が期待できる。