Mechanism and generality of promoting cellular differentiation associated with a reduction in cell attachment area

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K12815
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Yasunaga Mayu 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (70712181)
• Co-Investigator(Kenkyū-buntansha): 廣瀬 志弘 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (80415736) ; 伊藤 敦夫 国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (30356480)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 表面形状 / ジルコニア / 間葉系幹細胞 / 骨分化 / 細胞接着

Outline of Final Research Achievements

Recently, surface microstructures of the scaffolding have been regarded as a key regulating factor for cellular differentiation of stem cells because they affect the cellular event, including cell proliferation and differentiation. Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells, associated with a reduction in the cell attachment area. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat adipose-derived mesenchymal stem cells, associated with a reduction in the cell attachment area. Further studies are required to clarify the mechanism of promoting osteogenic differentiation associated with a reduction in the cell attachment area.

Free Research Field

生体材料

Academic Significance and Societal Importance of the Research Achievements

一般的に細胞接着が弱い場合、細胞状態が悪い、足場材が不適といったマイナス評価を受ける。申請者の見出した低接着幹細胞程、骨分化能が高いという現象はこれまでの常識とは異なるものであるため、本研究の学術的意義は高い。低接着細胞における分化促進メカニズムが明らかになれば、細胞機能を向上させる培養方法や生体材料の開発に繋がると期待される。また再生医療分野において、初期細胞接着形態を指標にした幹細胞の品質評価方法としての応用も期待できる。