2021 Fiscal Year Final Research Report
Research and development of a multicellular spheroid formation system with controllable cell ratio
| Project/Area Number |
19K12852
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90130:Medical systems-related
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| Research Institution | Fukuoka Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 医用ロボット / 再生医学 / スフェロイド / 細胞構造体 |
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| Outline of Final Research Achievements |
This research group has established a technology for producing a large three-dimensional cell constructed using only the cell. In the production of vascular and cardiac cell constructs, the use of the multicellular spheroids with mixture of fibroblasts and endothelial cells has been found to be effective. However, it is not easy for technicians to do this. Additionally, it is necessary to suppress variation factors that occur from human operation by using automation, improving data accuracy, and obtaining reproducible spheroids. Therefore, the aim of this study was to develop a multicellular spheroid formation system for the fabrication of cell structures for regenerative medicine. As a result of the research, it became possible to create multicellular spheroids with an arbitrary cell ratio using the developed system. Additionally, it was found that the multicellular spheroids obtained varied according to the different timing of dispensing.
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| Free Research Field |
生体医工学
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| Academic Significance and Societal Importance of the Research Achievements |
作業者の専門的な知識や技術に依存せず，コンタミネーションが発生しないクリーン環境下で，目的の細胞比率を有した多細胞スフェロイドを作製できるようになった．さらに，作業工程が異なると，多細胞スフェロイドの時間経過に伴う変化や内部の細胞の状態が異なる事を見つけることができた．これらの情報は，培養工程の自動化における作業方法の検討等に役立つと考えられる．本研究成果は，再生医療用細胞構造体の高度化および多様化に応用できるだけでなく，研究サイクルを向上させ，力学的および機能的な観点からの多細胞スフェロイドの特性の解明にも繋げられると考えられる．
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