Development of an analysis model for harmful side effects of synthetic cannabinoids using neuromuscular co-culture.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K12874
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90140:Medical technology assessment-related
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: Tomiyama Kenichi 国立研究開発法人国立精神・神経医療研究センター, 精神保健研究所 薬物依存研究部, 室長 (20584064)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 合成カンナビノイド / NG108-15 / RD / C2C12 / 細胞毒性 / 神経筋共培養モデル

Outline of Final Research Achievements

In this study, to elucidate the mechanism of synthetic cannabinoid-induced cytotoxicity and seizure, we attempted to generate a NG108-15, C2C12 and RD cell lines or their combinations for a neuromuscular co-culture model. Each cell lines induced cytotoxicity in a synthetic cannabinoid concentration-dependent manner, and the mechanism was found to be mediated by CB1 receptors. Although a neuromuscular co-culture model combining differentiated NG108-15 and differentiated C2C12 or RD cell line was created, the skeletal muscle action potentials under the neuronal control was not achieved.

Free Research Field

神経薬理

Academic Significance and Societal Importance of the Research Achievements

合成カンナビノイドは世界的に乱用されている薬物であり、無数の化合物が存在する。それらを動物で個別に評価することは困難であり、培養細胞を用いたスクリーニング法の確立は有害作用の予測を行う上で大変意義がある。特に横紋筋融解症のような、重度の障害発症の予測については、動物愛護の観点からも培養細胞での実施が期待される。