2023 Fiscal Year Final Research Report
Studies on fluctuation of biofilm formation of Listeria monocytogenes
| Project/Area Number |
19K14024
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 08030:Family and consumer sciences, and culture and living-related
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| Research Institution | Kamakura Women's University (2023)
National Institute of Health Sciences (2019-2022) |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2024-03-31
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| Keywords | Listeria monocytogenes / バイオフィルム / 環境適応 / 不均一性 / 細胞表面特性 / 菌体脂肪酸組成 |
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| Outline of Final Research Achievements |
Biofilm formation by Listeria monocytogenes in food manufacturing environments results in the contamination of food and is therefore a cause for concern. This study aimed at understanding the fluctuation of cells formed biofilm of L. monocytogenes through to clarify the characterizations of the sub-populated cells from biofilm. The biofilm-forming cells of L. monocytogenes serotype 1/2a strains were observed the morphological variation, and it was suggested that this variation may be due to associate with the adhesive strength and the cell surface structure. Additionally, it was also suggested that the influence of the variation in the fatty acid composition of the cell may be a factor in the change of cell surface properties.
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| Free Research Field |
食品衛生学
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| Academic Significance and Societal Importance of the Research Achievements |
食品製造環境におけるL. monocytogenesのバイオフィルム形成は食品への持続汚染を誘起する一因と目されており、その制御が必要とされている。本研究を通じて、形成されたバイオフィルム中の付着強度別細胞群衆における細胞特性を分子代謝的かつ細胞構造的に比較し、当該形成に伴うゆらぎの一端を明らかにすることができた。本成果は、集団微生物学的かつ環境生態学的観点からの当該形成機構の理解につながると共に、バイオフィルム形成制御に資する重要な学術知見となり、将来的に食品衛生分野に加えて医学・環境微生物学分野においても高い意義をもたらすと考えられる。
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