2022 Fiscal Year Annual Research Report
Multifunctional Au/Silk Nanoparticles Prepared by Facile Co-Precipitation Method and Evaluation for Delivery, Imaging and Targeting in Combination Cancer Therapy
| Project/Area Number |
19K15388
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | silk protein / gold / hybrid / nanomaterials / drug delivery / cancer therapy / photothermal therapy |
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| Outline of Annual Research Achievements |
Varying the contents of Au in Au/silk nanoparticles shows negligible change in the drug loading efficiency. By increasing the irradiation time or Au content in the Au/silk nanoparticles, we can achieve drug release of nearly 90% at pH7, which is relatively slow with silk alone. It is suggested that the boosts in drug release were mainly driven by enhancing the dissociation of the absorbed drug due to increase of temperature, while the thermal hydrolysis of silk protein play a supporting role. Photothermal effect of Au nanoparticles leads to low- or high-grade hyperthermia (43 and 45°C, respectively) and induces cell apoptosis when combined with anticancer drugs. This strategy has been confirmed by in vitro experiments with two types of anticancer drugs, DOX and MitC, on 4T1 cells line.
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