2019 Fiscal Year Research-status Report
Living skin-section reconstructed on a microfluidic chip
| Project/Area Number |
19K15415
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| Research Institution | The University of Tokyo |
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Principal Investigator |
聶 銘昊 東京大学, 大学院情報理工学系研究科, 助教 (40816485)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | organ-on-a-chip / skin section / epidermis culture |
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| Outline of Annual Research Achievements |
The aim of this research project is to build a type of organ chip with a reconstructed thin piece of skin section.
At the time of this report, the design and prototyping of the microfluidic chip have been finished. First, dermis layer, consisting of normal human dermal fibroblasts (NHDF) and collagen/alginate/fibrin can be recontrcuted.
NHDFs can be injected during the formation of the dermis and can proliferate within the formed dermis. Second, the design of the microchannels in the chip was optimized and the surface of the microchannels was properly coated to avoid unwanted cell loss during the seeding of normal human epidermal keratinocytes (NHEK). Upon culture, the NHEK formed into multicellular tissue with features resembling the epidermis in-vivo.
Patent of the chip was filed.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The formation of a well-differentiated full-thickness epidermis section is an important check point in this project. At the time of this report, the differentiation of the epidermis still need some further optimization, which was anticipated at the beginning of this project. Despite the difficulties, progress have been made by experiments to find out the best condition for epidermis culture.
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| Strategy for Future Research Activity |
In the final year of this research, first, the culture of epidermis will be further optimized to yield a fully cornified epidermis with multiple layers replicating the in-vivo epidermis; then, the chip will be used to evaluate the barrier functions of epidermis by investigating the the permeability of molecules with epidermis treated using a various kind of skin corrosive drugs. Meanwhile, summary of the work will be made and submitted to a scientific journal depending on the progress achieved.
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| Causes of Carryover |
The equipment choice for the perfusion culture of the organ chip was changed; the chosen one was actually cheaper than the initial planned one.
In the next year, in addition to the initial plan, I plan to purchase more types of cells and relative culture mediums to further compare the performance of them in order to establish a better protocol for epidermis differentiation.
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[Journal Article] The bioprinting roadmap2020
Author(s)
Wei Sun, Binil Starly, Andrew C Daly, Jason A Burdick, Jurgen Groll, Gregor Skeldon, Wenmiao Shu, Yasuyuki Sakai, Marie Shinohara, Masaki Nishikawa, Jinah Jang, Dong-Woo Cho, Minghao Nie, Shoji Takeuchi, Serge Ostrovidov, Ali Khademhosseini, Roger D Kamm, Vladimir Mironov, Lorenzo Moroni and Ibrahim T Ozbolat
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Journal Title
Biofabrication
Volume: 12
Pages: Number 2
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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