Real-time three-dimensional temperature measurement of photothermal microbubbles by using quantum sensors

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K15422
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 28050:Nano/micro-systems-related
• Research Institution: National Institutes for Quantum Science and Technology (2020-2021); Osaka City University (2019)
• Principal Investigator: Nishimura Yushi 国立研究開発法人量子科学技術研究開発機構, 量子生命科学研究所, 博士研究員 (00824434)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 蛍光ナノダイヤモンド / ODMR / 温度

Outline of Final Research Achievements

We have developed a wide-field ODMR microscope that enables simultaneous temperature measurement at multiple points, and analyzed its characteristics in detail. The ODMR spectra obtained using confocal detection and widefield detection were compared with the same fluorescent nanodiamonds, we found that the ODMR is deeper with confocal detection than with widefield detection. The effects of pixel saturation and positional drift on wide-field ODMR detection were investigated to study measurement artifacts and sensitivity degradation. From this system characterization, we adjusted the measurement parameters and performed ODMR measurements of multiple fluorescent nanodiamonds in living HeLa cells.

Free Research Field

ナノバイオ

Academic Significance and Societal Importance of the Research Achievements

本研究は，微小領域の高速・高精度な温度マッピングを可能にしようとするものである．共焦点検出とワイドフィールド検出はこれまで別々に扱われてきたが，1台の顕微鏡に組み込むことで初めて各々の特性を詳細に比較することができた．また，細胞内温度計測という観点においてはワイドフィールド検出の有用性を示すことができた．本研究成果は，カメラによる生きた細胞のリアルタイム大面積スピンベース測定の開発に役立つと期待される．