Preparation of high-sensitive sensors toward reactive oxygen species utilizing signal amplification based on the structure of a polyrotaxane

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K15529
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 33010:Structural organic chemistry and physical organic chemistry-related
• Research Institution: University of Tsukuba
• Principal Investigator: Chiba Yusuke 筑波大学, 数理物質系, 助教 (10823718)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ロタキサン / 蛍光センサ

Outline of Final Research Achievements

Fluorescence sensors are useful to analyze distribution of chemical species in living cells. Reactive oxygen species make important rolls in signal transduction. Due to the high reactivity and small abundance in living cells, the development of high-sensitive sensor for reactive oxygen species has been required. We envisageｄ that polyrotaxanes are effective to prepare high-sensitive fluorescence sensors utilizing signal amplification based on the characteristic structure comprised of rings and an axle. In this work, We successfully synthesized a novel [2]-rotaxane, and investigated its fluorescence sensing ability toward a fluoride anion. It has been confirmed that the fluorescence intensity intensively increased upon addition of a fluoride anion to the [2]-rotaxane. The structure of the [2]-rotaxane is therefore effective to prepare high-sensitive fluorescence sensors.

Free Research Field

超分子化学

Academic Significance and Societal Importance of the Research Achievements

従来の蛍光センサはターゲット化学種と蛍光センサとの1:1反応を用いるため感度には限界がある。本研究では、ターゲット化学種1分子から複数の蛍光センサの蛍光がONとなりうるポリロタキサンを提案し、その基本骨格となる[2]-ロタキサンに関して蛍光センシング能を検討したところ、ロタキサン構造の分解による蛍光増強を確認した。この結果はポリロタキサン構造を用いることで、ターゲット化学種1分子の刺激を増幅可能な高感度センサへの展開が期待できる。