2019 Fiscal Year Research-status Report
Development of Alternating Current Resistive Pulse Sensing Using Transport-Induced-Charge Theory
| Project/Area Number |
19K15600
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| Research Institution | The University of Tokyo |
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Principal Investigator |
徐 偉倫 東京大学, 大学院工学系研究科(工学部), 講師 (50771549)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | Alternating current / Electrokinetic pumping / Nanofluidics / Nanopore / Biosensing |
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| Outline of Annual Research Achievements |
We have made tremendous progress both experimentally and theoretically. The development of volumetric and gravimetric methods has been conducted for flow rate measurements in nanopores. We obtained the velocity of a dye droplet in a millimeter-scale capillary silica tube connecting to the nanopore reservoir to estimate the flow rate in the nanopores. On the other hand, the flow rate was directly measured by weighting the output solution from the nanopore tank, using a high precision analytical balance. Theoretically, we optimized the operation conditions using numerical simulation. It was found that there exists a critical size of nanopore (~ 30 nanometers) giving the highest flow rate for a fixed total cross-sectional area. These results can guide our experimental work in the second year.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
There are a few reasons making our progress be ahead of the plan. First, for the ultra-low flow rate measurement experiments, we borrowed the experience from Prof. Yutaka Kazoe at Keio University, who has recently developed a novel method to accurately measure the flow rate in nanochannels. Given that we have mutual interests in nanofluidics, we have started collaboration for several topics. Another reason is that we have employed an excellent postdoctoral researcher coming from Tsinghua University in China, who has extensive experience in electrokinetics not only for experiments but also modeling. His join to the lab remarkably improves the quality of our work. I cannot ignore the tremendous efforts from our excellent students. It is indeed the teamwork resulting in the fruitful outcome.
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| Strategy for Future Research Activity |
In the second year, our plan is to observe direct evidence in the experiments to verify the proposed transport-induced-charge theory. Although in the first year, we have constructed the experimental systems and used theoretical methods to predict the optimal conditions for measurements, the sudden arrival of the unprecedented COVID-19 pandemic has given some uncertainties to our schedule due to the temporarily shut down of all the experimental activities. If the situation allows us to perform experiments in the coming months, we will execute the plan and complete the experimental work. However, in the meanwhile, we are theoretically investigating thermal effects on transport-induced-charge phenomena. If the pandemic situation continues, we will shift the focus onto this theoretical work.
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[Presentation] Two-Dimensional Nanopore DNA Sequencing by Diffusion Current Measurements2019
Author(s)
Wei-Lun Hsu, Yuji Nakagawa, Daisuke Egusa, Soumyadeep Paul, Ya-Lun Ho, Jean-Jacques Delaunay, Zhen Gu, Yi-Lun Ying, Yi-Tao Long, Eiji Abe, Yoshihiro Iwasa and Hirofumi Daiguji
Organizer
2019 Materials Research Society (MRS) Fall Meeting
Int'l Joint Research
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[Presentation] Isothermal DNA Sensing Using Diffusion Current2019
Author(s)
Wei-Lun Hsu, Soumyadeep Paul, Zhen Gu, Ya-Lun Ho, Jean-Jacques Delaunay, Yi-Lun Ying, Yi-Tao Long and Hirofumi Daiguji
Organizer
The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)
Int'l Joint Research
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