Development of high-throughput system for extraction of intracellularly localized RNAs

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K15601
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 34020:Analytical chemistry-related
• Research Institution: Kanazawa University
• Principal Investigator: ZHOU YUANSHU 金沢大学, ナノ生命科学研究所, 特任助教 (60758556)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 細胞内回収

Outline of Final Research Achievements

RNA transported from the nucleus of a neuron to its dendrites, which divide like branches, are expected to be involved in memory formation. However, functional analysis of localized RNA is difficult due to difficulties in local access methods to target sites and throughput of analysis. In this study, dynamic imaging of neurons and recovery of local RNA were realized by using a multifunctional nanopipette developed by the applicant, which can both measure the non-invasive shape of the cell surface and recover a very small amount of liquid material.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究の手法はナノスケールで生細胞の形状変化を非標識・非接触で取得可能であり、他の技術では計測困難な細胞の局所的変化を可視化できる。また、ナノピペットを探針に用いるため、局所的な薬剤投与や細胞質の回収が可能である。その成果の発展として、RNAとともに回収したタンパク質の質量分析により、RNA輸送のメカニズムと機能の解析が可能になる。さらに、RNAと記憶形成の関連性が解明できれば、RNAの神経細胞間移植による記憶の再現という研究分野の開拓が期待できる。