Construction of polymer vesicles with defined structure

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K15643
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 35020:Polymer materials-related
• Research Institution: Kyoto University (2020); Institute of Physical and Chemical Research (2019)
• Principal Investigator: Fujita Seiya 京都大学, 工学研究科, 特定助教 (30824007)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: ペプチド / ベシクル / タンパク質

Outline of Final Research Achievements

The encapsulation efficiency of guest molecules in polymer vesicles has been extremely low at a few percent.Therefore, there is required for the construction of polymer vesicles with a high encapsulation efficiency. In this study, we constructed novel vesicles with high encapsulation efficiency by the anionic peptide Mal-Sar4-Glu12 having oligosarcosine is mixed with various cationic peptides. Vesicular assemblies were formed in the mixture solution of Mal-Sar4-Glu12 and Lys12. Moreover, when the encapsulation efficiency of the protein was evaluated, it had a high encapsulation efficiency of about 18%.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

これまでのポリマーベシクルのゲスト分子の内包率は数%と非常に低かった。本研究では内包率の高い新規PVの構築を目指して、Mal-Sar4-Glu12とLys12を合成し、これらを混合することでの18%程度と高い内包率を有するベシクルの調製に成功した。これまでにない内包率の高いベシクルを調製でき、今後のポリマーベシクルの設計の幅を大きく上昇することができたと考えられる。また、ナノリアクターへの応用が考えられるため非常に高い社会的意義を有する。