The development of peptide tethered cNDI cluster for specifically targeting telomeric tetraplex DNA

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K15700
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: ZOU TINGTING 九州工業大学, 大学院工学研究院, 特任助教 (30828637)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: G-quadruplex / naphthalene diimide / peptide linker / cluster

Outline of Final Research Achievements

In this study, (1) we firstly employed peptide as the linker part to construct novel ligand cluster, cyclic naphthalene diimide was adopted as the base G4 ligand unit, which showed preference for G-quadruplex recognition; several pep-cNDI dimers were newly synthesized; (2) then we confirmed that pep-cNDI dimers showed enhanced preference for recognizing and stabilizing mono G-quadruplex than cNDI monomer, within the determination by CD-based Tm measurement and ITC-based binding affinity assay; (3) finally we adopted ESI-MS to analyze the binding property of pep-cNDI dimers with G-quadruplex, the results suggested that two pep-cNDI dimers prefer to bind to one G-quadruplex structure and form a much more stable complex

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

We firstly adopted peptide as linker part for constructing G4 ligand cluster, and confirmed the high potentiality of peptide as linker, which could enrich the development of new group of G4 ligands.