Method for efficient synthesis of chemically functionalization proteins and application for biology research

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K15705
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
• Research Institution: Nagoya University
• Principal Investigator: Oishi Shunsuke 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (80707795)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: タンパク質化学 / ペプチド化学 / ケミカルバイオロジー / 有機合成化学

Outline of Final Research Achievements

We developed a Late-Stage Functionalization method for chemically synthesized proteins, aiming to systematically and exhaustively synthesize various uniformly structured modified proteins. The distinguishing feature of this study is the ability to obtain modified proteins with completely uniform structures by chemically synthesizing proteins and controlling the reactivity of side chain functional groups.
We succeeded in fluorescently modifying a plant cysteine-rich protein, and conducted bioactivity assays and bioimaging experiments.

Free Research Field

有機合成化学

Academic Significance and Societal Importance of the Research Achievements

タンパク質の合成では、高純度のタンパク質が得られる反面、しばしば面倒で時間がかかるプロセスである。特に、化学修飾を組み込む場合は、合成初期段階で修飾構造を導入する必要があるため、全合成プロセスを繰り返すことが必要となる。本研究ではLate-Stage Functionalization法により、迅速に修飾タンパク質を化学合成する方法を開発した。