Role of reactive oxygen species in the intestinal tract caused by the interaction of food ingredients

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K15777
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Hoshi University
• Principal Investigator: Iwasaki Yusuke 星薬科大学, 薬学部, 准教授 (10409360)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 食品成分 / 相互作用 / 酸化ストレス

Outline of Final Research Achievements

Active ingredients may be ingested through foods, and they can cause several interactions in the human body. In this study, we examined the chemical reactions between hydroxycinnamic acids, a group of polyphenols, and metal ions in artificial intestinal fluid. Caffeic acid (CaA) and sinapic acid (SA) reacted with copper ions under artificial intestinal fluid conditions and produced new compounds. Relative to the initial compounds, CaA and SA derivatives produced by condensation exhibited an increased antioxidant and a decreased prooxidant activity. On the other hand, to evaluate the biological effects caused by the interaction between epigallocatechin gallate and metal ions, metabolomics analysis using GC/MS/MS was performed. This study revealed a new food ingredient interaction pattern in which new compounds are produced under biological conditions.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

腸管内における食品成分の相互作用によって慢性的に生じた活性酸素種（ROS）が、腸内細菌や腸管基質に対してどのような生理的役割を与え、生体内の恒常性や疾患発症に寄与しているかについては、不明な点が多かった。本研究の結果から、食品成分の相互作用によってROSが産生されることが示され、その過程で新しい化合物が生成されることが明らかとなった。本研究で得られた結果から、腸管内をターゲットとしたROSのバランスを調節する新薬の開発や予防のための機能性食品を提案する上で、重要な知見になり得ると考えられる。