Elucidation of the regulation of adipocyte differentiation by RNA binding protein, Mex-3B

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K15804
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 38060:Applied molecular and cellular biology-related
• Research Institution: The University of Tokyo
• Principal Investigator: Kamoshida Yuki 東京大学, 定量生命科学研究所, 特任研究員 (50835759)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 脂肪細胞分化 / RNA結合タンパク質 / PPARγ / C/EBPβ

Outline of Final Research Achievements

We previously found that RNA binding protein Mex-3B deficient mice are resistant to obesity and long-lived. During the process of this study, we discovered that Mex-3B deficient cells are less capable of differentiation into adipocytes.Therefore, in order to elucidate the mechanism of regulation of adipocyte differentiation by Mex-3B, we identified mRNAs that bind Mex-3B protein exhaustively and analyzed them.In the result, we found that Mex-3B protein binds with PPARγ and C/EBPβ mRNA. Moreover, Mex-3B protein regulate PPARγ expression in mRNA levels, whereas regulate C/EBPβ expression in protein levels.

Free Research Field

応用細胞生物学

Academic Significance and Societal Importance of the Research Achievements

高齢化社会が進行している現代日本において、健康寿命の延長法の解明は急務である。これまでに我々はMex-3Bの欠損マウスがやせ型ならびに健康寿命の延長という表現型を呈していること見出しており、その分子機構を明らかにすることは、健康寿命の延長法の解明につながる。また、脂肪細胞におけるMex-3Bの機能を明らかにすることで、寿命制御においてMex-3Bが果たす役割を明らかにすることが期待できる。