Identification of causal genes controlling the flowering period and numbsers in Nelumbo nucifera.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K15819
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Tokyo Kasei Gakuin University
• Principal Investigator: Ishizuna Fumiko 東京家政学院大学, 現代生活学部, 准教授 (40772281)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: ハス / Nelumbo nucifera / QTL解析 / RAD-Seq ジェノタイピング / DNAマーカー選抜育種法

Outline of Final Research Achievements

A F2 population consisting of approximately 150 individuals was generated. Traits of flowering period, flower colors, petal numbers, leaf blade size, petiole heights were evaluated for multiple years and it was confirmed differed within population, therefore, this F2 population was useful for this research. To identified a putative candidate gene of traits, preliminary QTL analysis was performed using the results of RAD-seq genotyping and linkage mapping by RAD-seq. Positive results were obtained, for example, for petal color (red = 1, white = 0), a very effective QTL was detected on the long arm of chromosome 4.

Free Research Field

39010：遺伝育種科学関連

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、ハスのDNAマーカー選抜育種法の確立に寄与し、ハス育種の効率化および利用目的に合わせた育種への貢献が期待できる。また、ハスの研究のみならず他の植物種の育種研究にも応用可能な基礎的知見が得られると考えられる。将来的には本研究で用いている解析集団の地下茎を分譲し、公的な実験材料として提供するという方向での貢献も可能であると考えている。