Three-dimensional anatomy of chloroplasts in mesophyll tissues related to carbon dioxide in leaf tissues of Grasses

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K15823
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39020:Crop production science-related
• Research Institution: Nagoya University
• Principal Investigator: Oi Takao 名古屋大学, 生命農学研究科, 助教 (60752219)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: イネ科作物 / C４光合成 / 葉緑体 / 環境ストレス / 三次元観察 / 卓上SEM / マイクロX線CT

Outline of Final Research Achievements

The three-dimensional reconstructed models of mesophyll cells and their chloroplasts of rice (C3 plant) and finger millet (C4 plant) were shown by serial-sectioning light microscopy and serial-sectioning electron microscopy (using desk-top scanning electron microscope). The intercellular airspaces were also analyzed three-dimensionally, and the surface area of mesophyll cells and chloroplasts facing intercellular airspaces were measured.

Free Research Field

植物形態

Academic Significance and Societal Importance of the Research Achievements

本研究を通じて連続切片-卓上SEM法による三次元再構築法が確立した．これにより，組織の内部構造を細胞・細胞内小器官のレベルで３D解析することが可能となった．本手法で使用する卓上機は大型で高額な電子顕微鏡よりも導入し易く，操作方法も簡便であるため，学生やこれまで電子顕微鏡を使用したことのなかった初心者にも利用し易い利点もあり，今後の普及・展開が期待される．