Elucidation of pathogen recognition system mediated by bacterial wilt resistant protein

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K15847
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Okayama University
• Principal Investigator: Nakano Masahito 岡山大学, 環境生命科学研究科, 特別研究員(PD) (60756708)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 青枯病 / 病害抵抗性 / エフェクター

Outline of Final Research Achievements

Ralstonia solanacearum is the causal agent of bacterial wilt in solanaceous crops. Nicotiana plants recognize RipB effector from R. solanacearum and induce defense responses. In this study, we aimed to identify a novel host factor involved in RipB recognition and to clarify the virulence mechanism of R. solanacearum that inhibits the recognition. By using virus-induced gene silencing, we found that PsbO and PsbP play important roles as factors involved in RipB recognition. In addition, we identified R. solanacearum effectors that inhibit RipB recognition and found several host factors that interact with the effectors using yeast two-hybrid screening.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

青枯病菌の宿主範囲は広く、その病原性機構は不明な点が多い。そのため、本菌に対する防除方法を開発する上で大きな障壁となっている。本研究では、青枯病菌エフェクターの機能解析を通して、青枯病菌が植物からの認識を回避する病原性分子機構を解明した。また、青枯病抵抗性の誘導に関わる情報伝達経路の一端が明らかになり、病害防除方法を確立する上で有益な知見が得られた。