2020 Fiscal Year Final Research Report
The search study of the molecular mechanisms about the autonomous myofiber type determination by myogenic stem cells.
| Project/Area Number |
19K15959
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 42010:Animal production science-related
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| Research Institution | Kyushu University (2020)
Hokkaido University (2019) |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | 筋幹細胞 / 衛星細胞 / 筋タイプ / 初期決定機構 / 単一筋線維 / 培養基質の弾性率 |
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| Outline of Final Research Achievements |
We focused on resident myogenic stem cells (satellite cells) are attracting attention for their novel roles in myofiber type regulation. When satellite cells were separately isolated from the slow fiber-abundant muscles or the fast-type muscles, each cell highly synthesized semaphorin 3A or netrin-1 for autonomous myofiber type determination. However, the molecular mechanisms how did each muscle derived-satellite cells acquire their characters were uncertain. We hypothesized that the two cases would be the key contents for the specification-acquirement mechanisms. First, satellite cells can maintain the unique potentials according to their host myofiber even though the post-isolation. Second, satellite cells may be affected the physical stimulations like elasticities of each culture substrate. Upon our results, we predict that satellite cells isolated from each myofiber, keeping their own specification, would be responsible for the autonomous myofiber type commitment.
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| Free Research Field |
筋細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
食肉の質を向上させるためには、骨格筋の筋タイプ（遅筋型および速筋型で大別される）を制御する生産方法の開発が求められる。これまでに、筋タイプは成熟した筋線維に接着する運動神経刺激の支配下にあると考えられてきたが、各筋タイプ特異的な筋線維に局在する衛星細胞は自律的に筋管の筋タイプを制御する能力を有することが明らかとなっている。本機構は物理的刺激などの外的要因を受けずに維持される可能性があるため、それぞれの筋タイプに特異的な衛星細胞をターゲットとした筋肥大誘導技術開発によって、容易に筋タイプを変換する新技術開発に繋がることが期待される。
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