Development of cell isolation technology by elucidating the mechanism of angiogenesis of mesenchymal stem cells

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16018
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42030:Animal life science-related
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Watanabe Tomoko 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (70827980)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 間葉系幹細胞 / 血管新生

Outline of Final Research Achievements

This study aimed to elucidate the secreted factor/transcription factor network of MSCs with high angiogenic potential and its application to cell isolation systems. Recently, therapeutic effects of MSCs on ischemic diseases have been reported, and research is underway. However, the angiogenic potential of MSCs varies from strain to strain. In this study, we addressed the following issues: 1) identification of MSCs with high angiogenic potential using an angiogenic potential evaluation system, 2) identification of angiogenesis-related factors by comprehensive analysis, and 3) application to cell separation systems. By the end of this fiscal year, we have established an evaluation system for angiogenic potential using culture supernatants and succeeded in identifying signaling-related factors involved in angiogenesis by proteome analysis. Currently, preparations are underway to develop a technology for the isolation of MSCs with high angiogenic potential.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

本研究の目的は高血管新生能MSCの分泌因子／転写因子ネットワークを解明することである。具体的にはin vitro評価系を用いて血管新生能の高い細胞株を選出し、発現の高い因子をマイクロアレイ解析により選出する。転写因子群やシグナル関連因子を高血管新生能予測マーカーとして同定し、血管新生機能の検証を行う。当研究室の先行研究において、虚血状態の際にIGFやVEGFリガンドを介したPI3K/AKTシグナルが抑制されている可能性が示唆されていた。そこで高血管新生能予測マーカー候補とPI3K/AKTシグナル関連因子の関連について検証を進める。また過剰発現や機能欠損実験を行い、血管新生能への影響を検証する。