Thermo-sensing Mechanism of Heat Shock Factor 1 through Comparative Biology

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16046
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 43010:Molecular biology-related
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Sakamoto Joe 基礎生物学研究所, 生命熱動態研究室, 特任助教 (80804145)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 熱ショック応答 / HSF1 / 温度 / 比較生物学 / メダカ / ヒラメ

Outline of Final Research Achievements

Heat shock response is a biological system for the protection from heat stress. On the Earth, there are varios temperature environment in every different region. Organisms adapt to and live in these temperature environment. Threfore, there is a possibility that the threshold temperature at which heat shock response is induced is different depending on the environments. In the current study, I focused on heat shock transcription factor (HSF) 1, one of the master regulator of heat shock response, and use medaka and flounder ,which inhabitats in the lower temperature than medaka, for the purpose of the comparison of HSF1 thermal property. I cloned hsf1 gene from both medaka　and flounder, and proceeded to construct the evaluation systems to analyze temperature property.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

温度は生物の生存にとって非常に重要な要素の一つである。熱ショック応答は古くから研究されているが、生物の生育環境温度の違いと熱ショック応答の関連について比較生物学的な観点から進められた研究は少ない。近年、温度センサーであるTRPチャネルがノーベル賞を受賞するなど、生物と温度の関わりが注目を浴びている。そのような中で、本研究の成果は生物の環境温度適応戦略に新たな知見をもたらしうるものであると言える。