Regulatory mechanism of CAT-tailing by Vms1 and role of an endogenous sequence for RQC

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K16052
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 43020:Structural biochemistry-related
• Research Institution: Tohoku University
• Principal Investigator: Izawa Toshiaki 東北大学, 薬学研究科, 助教 (60837871)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: タンパク質品質管理 / リボソーム / RQC / Vms1

Outline of Final Research Achievements

Aberrant proteins produced by ribosome stalling can be modified by the addition of CAT-tail. However, the molecular mechanism for regulation of CAT-tailing is not well understood. In this study, using yeast cells, I analyzed the regulatory mechanism of CAT-tailing by focusing on a key factor Vms1.
A recent study has demonstrated that yeast cells have a protein that can cause ribosome stalling. However, why yeast cells have preserved such stalling sequence is not well understood. This study aimed to uncover its physiological role.

Free Research Field

細胞生物学、生物化学

Academic Significance and Societal Importance of the Research Achievements

異常なタンパク質の蓄積は細胞機能の破綻を引き起こし、様々な疾患の原因にもなる。健康な細胞では通常、異常タンパク質を除去する品質管理機構が備わっており、それにより細胞の恒常性が保たれている。本研究では、最近新たに見つかった品質管理機構であるリボソーム関連品質管理（RQC）の分子機構について、その一端を明らかにした。本研究成果は、将来的に異常タンパク質の蓄積を起因とする疾患の発症機構を理解するための分子基盤になると考えられる。