Structural analysis of antibody IgG-FcgR complex using cryo-electron microscopy

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K16063
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 43020:Structural biochemistry-related
• Research Institution: National Institute of Health Sciences
• Principal Investigator: Kiyoshi Masato 国立医薬品食品衛生研究所, 生物薬品部, 研究員 (60754314)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 抗体 / FcγR / クライオ電子顕微鏡

Outline of Final Research Achievements

Higher-resolution structural analysis of the antibody-FcgR complex was performed using a cryo-electron microscope that can observe the structure of biomolecules in solution. After preparing the antibody-FcgR complex, it was found that it is important to set the conditions under which the proteins on the grid can avoid aggregation for structural analysis.

Free Research Field

生命科学

Academic Significance and Societal Importance of the Research Achievements

抗体や抗原抗体複合体とFcγRがどのような立体配座で結合しているか、その結合の分子メカニズムが明らかにするため、大腸菌を用いて、FcγRの細胞外ドメインの発現、精製を行い、抗体との複合体を形成させた後、クライオ電子顕微鏡を用いて構造解析を行った。調製した抗原分子を添加し、抗原、抗体、FcγRの三者複合体の構造解析を行った。