2021 Fiscal Year Final Research Report
Enzymatic synthesis and degradation of O-Mannose glycan
| Project/Area Number |
19K16064
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43030:Functional biochemistry-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 糖質加水分解酵素 / 糖鎖 / 配糖体 |
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| Outline of Final Research Achievements |
Muscle is a tissue that generates force through contraction and is an important organ in animals. Recently, it has been discovered that "O-mannose-type glycans" attached to membrane proteins of muscle cells are essential for muscle cell stabilization, and the enzymes involved in their biosynthesis have been clarified. On the other hand, how O-mannose-type glycans are degraded in necrotic myocytes was unknown. In this study, we aimed to establish an enzymatic synthesis method of O-mannose-type glycans independent of biosynthetic enzymes, with the ultimate goal of elucidating the metabolic pathway of O-mannose-type glycans, and found that there are many known enzymes that catalyze α-mannosyltransfer reaction.
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| Free Research Field |
生化学
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| Academic Significance and Societal Importance of the Research Achievements |
酵素のもっとも大きな特徴として「基質特異性（特定の化合物にしか作用しない性質）」があげられる。本研究において我々が発見した「α-マンノシル基転移反応を触媒する酵素」は，これまで「一般的なα-マンノシドには作用しない」と信じられてきた酵素群であった．本研究成果は，基質特異性に囚われない新たな酵素利用法の開発研究のきっかけとなるものであり，ひいては新たな機能性物質の生産などを通じて社会に還元されることが期待できる．
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