• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2020 Fiscal Year Final Research Report

Integrated structural analysis of clock protein KaiC in solution with small-angle scattering and analytical ultracentrifugation

Research Project

  • PDF
Project/Area Number 19K16088
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 43040:Biophysics-related
Research InstitutionKyoto University

Principal Investigator

Morishima Ken  京都大学, 複合原子力科学研究所, 助教 (40812087)

Project Period (FY) 2019-04-01 – 2021-03-31
Keywords超遠心分析 (AUC) / X線小角散乱 (SAXS) / 時計蛋白質
Outline of Final Research Achievements

In this study, I investigated the structure of the clock protein “KaiC” in solution, which plays a fundamental role in circadian clock system of a cyanobacteria. First, an integral analytical method of analytical ultracentrifugation (AUC) and small-angle X-ray scattering (SAXS), namely “AUC-SAXS”, was developed to obtain the correct scattering profile for KaiC. Subsequently, the three-dimensional structure of KaiC, which reproduces the scattering profile, were searched with coarse grained-molecular dynamics (CG-MD). As a result, it was found that terminal chains of KaiC behaved as intrinsically disordered region.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

本研究ではKaiCのKaiAおよびKaiBとの相互作用を理解するために重要な3次元構造の知見が得られた。今後は天然変性領域として振る舞う両末端鎖の運動に立脚した複合体解析によって、生物時計機構のより詳細な理解につながると期待される。
また、本研究で開発したAUC-SAXS法はKaiCだけに限らず一般的な生体高分子に対して普遍的に適用可能である。溶液中の凝集による影響はX線小角散乱実験において長年の問題であったため、これを解決する方法として幅広く応用されることが期待される。

URL: 

Published: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi