2020 Fiscal Year Final Research Report
The construction of large-scale microsatellite database and bioinformatics pipeline system
Project/Area Number |
19K16113
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 43060:System genome science-related
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Research Institution | Tokyo University of Agriculture |
Principal Investigator |
TANAKA Keisuke 東京農業大学, その他部局等, 助教 (60747294)
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Project Period (FY) |
2019-04-01 – 2021-03-31
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Keywords | マイクロサテライト / 次世代シーケンサー / DNAマーカー / データベース / 解析パイプライン / NGS / SSR |
Outline of Final Research Achievements |
This study was able to achieve the target of 500 samples via recruitment of genomic DNA for organisms that are in demand for microsatellite information through such as researcher community and SNS. In addition, it succeeded in obtaining data more efficiently and at half the cost than before by adopting a protocol using restriction enzymes and forked adapters in the experimental system of microsatellite detection. It also acquired sequence data from some public databases and detected microsatellite information. On the other hands, a tool that perform homology search with sequences on the database was implemented as computational polymorphism analysis.
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Free Research Field |
ゲノム生物学
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Academic Significance and Societal Importance of the Research Achievements |
これまで品種改良が遅れていた作物・園芸品種に対してDNAマーカー開発が容易となり、マーカー選抜による育種効率の向上や、現場証拠として鑑定が必要な植物個体の識別技術の発展につながること、さらにDNAマーカー開発への適用は、生態保全や遺伝資源の担保につなげられることにも期待される。また、本研究代表者が所属する東京農業大学 生物資源ゲノム解析センターが掲げる農学支援としても非常に有用なゲノムリソースになるといえる。
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