Identification of the novel factor of peroxisomal contact sites using a proximity labeling system

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K16130
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: Kumamoto University
• Principal Investigator: Yamamoto Masatoshi 熊本大学, 大学院生命科学研究部(医), 助教 (70802114)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: オルガネラ / コンタクトサイト / ペルオキシソーム / 小胞体 / オルガネラゾーン

Outline of Final Research Achievements

Eukaryotic cells contain various organelles compartmentalized by the intracellular membrane to maintain their identity and specialize biological processes. These organelles are not only individual entities but also communicated between other organelles to perform their appropriate function. Such inter-organelle communication is achieved by vesicular trafficking and membrane contact sites. However, the molecular mechanism to form membrane contact sites is not fully understood.
In this study, we established the in situ protein labeling method for peroxisomal membrane contact sites using modified engineered ascorbate peroxidase (APEX2). Moreover, this method could apply to the isolation of other membrane contact sites, and it will be a powerful tool for future "Organelle zone" research.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究は、細胞生物学における近年のトピックのひとつである「オルガネラ・ゾーン」研究これまでににおいて、これまで技術的に困難であったオルガネラ近接領域の単離精製を可能にする技術の確立に成功した。本研究ではペルオキシオームと小胞体の近接領域をモデルに実証を行ったが、原理的には様々なオルガネラ近接領域に応用可能であると考えられる。今後、様々なオルガネラ近接領域の形成機構解明に重要な役割を果たす事が期待される。