GPI transamidase complex regulates nuclear position

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16132
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: Tokyo Institute of Technology (2021); Rikkyo University (2019-2020)
• Principal Investigator: Kawaguchi Kohei 東京工業大学, 科学技術創成研究院, 特任助教 (10835515)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: GPI / 核膜

Outline of Final Research Achievements

The subunits of Drosophila GPI transamidase complex mutually stabilize each other. Especially PIGK subunit was required all other subunits for protein stability. Furthermore, PIGK degradation was mediated by endoplasmic reticulum-associated degradation (ERAD).
Among the subunits of the Drosophila GPI transamidase complex, PIGT, PIGS, PIGU, and GPAA1 localized to the endoplasmic reticulum and nuclear membrane, while PIGK localized only to the endoplasmic reticulum.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

GPIトランスアミダーゼ複合体のサブユニットの過剰発現や局在の変化は癌との関係性が示唆されている。本研究はそれらのサブユニットのタンパク質安定性や局在について詳しく明らかにしたものである。今後、これらの知見がGPIトランスアミダーゼ複合体のサブユニットが過剰発現した癌の発症メカニズムの解明の一助になる可能性がある。