2019 Fiscal Year Research-status Report
Elucidating regulatory mechanism of haustorium number using a model parasitic plant system
| Project/Area Number |
19K16169
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Cui Songkui 奈良先端科学技術大学院大学, 研究推進機構, 特任助教 (20712532)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | haustorium / parasitic plant / auxin / nutrient / phenolics |
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| Outline of Annual Research Achievements |
The proposed research aims to understand how a parasitic plant regulates the number of haustorium which is an essential organ infecting host plants. Three major plans have been proposed initially and the progress is stated as below.
1. The effects of auxin for haustorium formation has been examined. Auxin marker test was conducted in the mutant producing more haustoria than the wild type, showing positive correlation between auxin accumulation at root tip and haustorium formation.
2. The role of nutrients on haustorium formation was revealed. Environmental nutrients mainly play negative effects on haustorium formation, and the responsible elements that suppress haustorium formation have been identified.
3. The first round of mutant screening for identification of more haustorium mutants were completed in FY2019.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Three major plans proposed initially have been conducted as planned and the progress is rather undergoing smoothly without delay.
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| Strategy for Future Research Activity |
The mode of auxin in regulating haustorium formation needs to be investigated further. Based on observation of auxin markers in the more-haustoria mutant, measurement of auxin level at root tips in wide type and mutant is necessary and currently undergoing.
As key nutrient elements that suppress haustorium formation have been identified, the related genes that are responsive to environmental nutrients for the control of haustorium formation will be surveyed using transcriptome analysis.
For the mutant screening, the phenotype confirmation for the isolated mutants from M2 will be conducted in M3 generation. Phenotype-confirmed mutants will be backcrossed with the wild type and the F2 lines will be subjected to next generation sequencing for gene identification.
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| Causes of Carryover |
Small amount of grant remained left as most the required consumables and reagents were already purchased in the last fiscal year. The remaining budget will be added up to DNA sequencing cost in next fiscal year.
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