2019 Fiscal Year Research-status Report
Structural-Functional Development of the Olfactory System
| Project/Area Number |
19K16261
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| Research Institution | Kyushu University |
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Principal Investigator |
LEIWE MARCUS 九州大学, 医学研究院, 助教 (80722008)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | Olfactory Bulb / Development / Odour / Pruning |
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| Outline of Annual Research Achievements |
We began by assessing the olfactory responses generated in neonatal mice. We were able to reliably record M/T cell responses from mice ranging from P2 to the adult using 2-Photon Ca2+ imaging. We assessed the difference between P2 and P6 to see if the sensitivity of glomeruli were different. P2 mice were slightly more broadly tuned than P6 mice. However, the responses were much stronger at P6 perhaps meaning that, we were able to detect more smaller events rather than actually observing a change in odour tuning.
Progress was made in being able to alter the phase code of neonatal mice. With a global KO of DA cells the consistency of the phase code was decreased. We have constructed a Cre-specific Tetanus AAV vector to see if we can reproduce these mice in healthier mice.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Our odour tuning experiments have been slightly delayed as the increased sensitivity of mice as they age was not accounted for. As a result, I will try to replicate the findings at a lower concentration. In addition I will try to come up with another solution.
The construction of the AAV vector has taken too much time. However, now that it is constructed and confirmed to work. What remains is to 1) calculate the right amount of virus to inject to ensure that enough of the OB is covered. 2)Perform calcium imaging while recording the respiration to see if the phase code is alterred.
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| Strategy for Future Research Activity |
1) Trial the lower concentration of odours at P2 to P6
2) Record the changes in phase coding after the injection of pAAV-Flex-TeNT-p2A-mRuby3
3) Come up with an alternative strategy if point 1) fails.
4) Try to find a way to prevent the pruning of mitral cells and see if there is an effect
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| Causes of Carryover |
Had to concentrate on preparing papers, and struggled with the creation of the AAV virus vector. Will spend the rest in the following financial year
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