2020 Fiscal Year Final Research Report
Development of an all-optical neurophysiological method by using novel caged compounds
| Project/Area Number |
19K16291
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 46030:Function of nervous system-related
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | ケージド化合物 / 線虫 / カルシウムイメージング / 光制御 / C. elegans / 神経活動制御 |
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| Outline of Final Research Achievements |
To explore the relationship between neural activity and behavior, we aimed to develop the neurophysiological method that enables simultaneous optical manipulation and recording of neuronal activity in the nematode Caenorhabditis (C.) elegans by using yellow-light-activatable caged capsaicin. We created worm strains expressing TRPV1 and GCaMP6s in neurons and body wall muscles. Uncaging by yellow light with simultaneous monitoring of neuronal activity using GCaMP6s demonstrated that the uncaging triggers responses in sensory neurons, motor neurons, interneurons, and body wall muscles that are associated with behavioral changes of C. elegans.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，独自に開発した新規ケージド化合物を活用することによって，線虫の神経活動の人工操作と記録をすべて光学的に行い，線虫の神経活動と行動を対応づける手法を開発した．これは，神経回路の活動と行動を非侵襲的に直接結びつけることを可能とするもので，神経科学分野に新しい実験法を提供し，その発展に寄与できると期待される．また，神経回路機能の解析が光学顕微鏡下で容易に行えるため，本手法を活用することで神経伝達の障害から生じる神経・精神疾患，記憶学習障害などのメカニズム解明につながると考えられる．
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