2021 Fiscal Year Final Research Report
Study of electrochemical analysis for proteins via ECE reaction of SAR
| Project/Area Number |
19K16338
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
|
|---|
| Research Institution | Gifu Pharmaceutical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2019-04-01 – 2022-03-31
|
| Keywords | ラジカル付加反応 / プロトン電子共役移動反応 / 含硫タンパク質 / π共役軌道 / タンパク質立体構造 / スーパーオキサイド / ヒドロペルオキシラジカル / サイクリックボルタンメトリー |
|---|
| Outline of Final Research Achievements |
We observed the homogeneous-oxidation of some proteins by electrogenerated superoxide radical anion, using non-aqueous electrochemical measurements and quantum chemical calculations. Analysis of the current-voltage curve obtained by electrochemical measurement revealed that not only the oxidation of the protein surface but also the complexity inside the three-dimensional structure and the state of hydration greatly affects the reaction current value.
In the results, it was observed that the sulfur-containing protein showed a characteristic ESR spectrum, demonstrating that the two mechanisms, the radical adduct formation and the proton-coupled electron transfer between sulfur element and superoxide, may be the cause of the collapse of the three-dimensional protein structure.
|
| Free Research Field |
薬学、電気化学、計算化学、量子化学
|
| Academic Significance and Societal Importance of the Research Achievements |
当研究では、活性酸素種によるタンパク質の酸化が、タンパク質の凝集やアミロイド化に繋がるとの推論から、どのような化学反応メカニズムで起こりうるのか、その真理を探ることで、タンパク質の酸化理論の確立に貢献したい。これにより、タンパク質の新たな解析手法の開発、アルツハイマー症候群やパーキンソン病等の老齢性脳神経障害の治療薬開発などに寄与する情報を得られることから、学術的意義だけでなく、医療・薬学分野での応用に関連した社会的意義を有する。
|
