Development of bioluminescent probes for multi detection of biological components

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K16341
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: Kobe Pharmaceutical University
• Principal Investigator: Takagi Akira 神戸薬科大学, 薬学部, 助教 (00758980)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: ルシフェリン / 生物発光 / 遷移金属触媒 / 近赤外光

Outline of Final Research Achievements

The purpose of this research is development of near infrared bioluminescent substrate for the luciferase reaction. We achieved Sonogashira coupling of 2-iodobenzothiazole derivative with propargyl alcohol. We also found that trans selective hydrosilylation of propargyl alcohol underwent efficiently even the substrate has benzothiazole moiety.
We tried to transform from allyl alcohol to vinyl thiazoline via formation of acrylonitrile by using cinnamyl alcohol as a model compound.
We

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Academic Significance and Societal Importance of the Research Achievements

本研究成果はホタル発光基質の創製を目指し、ベンゾチアゾール環を含んだ基質の化学変換を行った。ベンゾチアゾール環は様々な反応に影響を与えると考えられたが、種々の検討を行い、遷移金属を用いたカップリング反応や置換基の導入反応について良好な収率で目的の分子を得ることに成功した。今後多様な置換基導入を達成することで複数の生体成分を同時に検出することが可能な生物発光基質の創製が可能となると期待される。