Elucidation of the production mechanism of adenosine deaminase 2 in breast milk and its role in intestinal mucosal tissue

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16355
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
• Research Institution: Hokkaido University of Science
• Principal Investigator: Ito Moeko 北海道科学大学, 薬学部, 講師 (60711827)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: アデノシンデアミナーゼ / 乳汁

Outline of Final Research Achievements

Breast milk contains a wide variety of bioactive substances and is directly or indirectly involved in the maturation of the newborn. In recent years, many novel proteins in breast milk have been discovered, but adenosine deaminase (ADA) isozymes, a group of enzymes known to irreversibly deaminate adenosine to inosine, have not been identified from comprehensive analysis. In this study, we detected ADA1 and ADA2 in crude purified whey samples which was obtained using the known chemical characteristics of ADA, respectively, and clarified changes in ADA activity during the first week postpartum.

Free Research Field

生物系薬学

Academic Significance and Societal Importance of the Research Achievements

母乳がADAアイソザイムを含有することが示唆され、初乳における高い活性は新生児期におけるADAの特異的役割が推測されるものである。ADA-related growth factorに属するADA2はヒトでは分布が限定的であるが、乳清中に継続して検出されたことでADA2の分布組織としての新たな知見が得られた。