Development of a novel biosensor using cells with hTAS2R and evaluation of bitterness of drugs

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16464
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 47060:Clinical pharmacy-related
• Research Institution: Mukogawa Women's University
• Principal Investigator: Kojima Honami 武庫川女子大学, 薬学部, 助教 (20779243)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: バイオセンサ / 苦味受容体

Outline of Final Research Achievements

In this study, we have worked on the development of a biosensor using cells with hTAS2R in order to bring it closer to the human taste receptor response.
Plasmid vectors with TAS2R10 and TAS2R14 gene sequences as hTAS2R were transfected into HEK293 cells, and stable cultured cells were established. Polyvinyl chloride, an existing polymer support, was used as the base of the sensor chip of taste sensor, and a support containing iron oxide was fabricated to enhance the conductivity of the support.

Free Research Field

臨床薬学

Academic Significance and Societal Importance of the Research Achievements

苦味物質によっては従来の味覚センサと反応しにくい物質も存在するため、ヒト官能試験の実施が不可欠であり、その試験結果と味覚センサの測定結果との相関性を考慮することが必要である。しかし、倫理的な観点から、ヒト官能試験を行うことのできない医薬品や新規開発医薬品などが存在するため、新規バイオセンサはヒト官能試験の代替法としても利用可能である。