2021 Fiscal Year Final Research Report
Three-dimensional analysis of ultrastructral changes of nuclear membranes and chromosomes during cell division using electron microscopy
| Project/Area Number |
19K16472
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 48010:Anatomy-related
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 細胞分裂 / 電子顕微鏡 / 三次元再構築 |
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| Outline of Final Research Achievements |
In order to elucidate three-dimensionally clarify the ultrastructural changes of the nuclear envelope, chromatin and chromosome that occur during cell division, HeLa cells were generated that express red fluorescent protein in lamins, an inner nuclear envelope-associated protein. Moreover, these cells were observed by using a confocal laser scanning microscope and the three-dimensional analysed using array tomography methods. As a result, it revealed that lamin were not exist in these cells during metaphase of cell division. Furthermore, it was shown that there is a fragmented membrane structure structurally similar to the nuclear envelope around the chromosomes gathered on the equatorial plane. These results were shown the possibility that the nuclear envelope did not completely disappear during metaphase of cell division in which is generally considered to disappear, and that it may remain.
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| Free Research Field |
電子顕微鏡学、顕微解剖学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、一般的には消失すると考えられている細胞分裂中期において核膜が断片化した膜として染色体周囲に存在している可能性を示すことができた。これまで細胞分裂前中期から後期においては存在していないと考えられていたことによりほとんど研究が行われてこなかったが、これらの細胞分裂期においても核膜の構造や機能の解析が必要であることを示す。今後、細胞分裂の全段階に渡る核膜の構造変化や機能が明らかにされることにより、核膜が関連する疾患の断定的な原因の究明、がん細胞の異常増殖の制御機構の解明へとつながる研究の足掛かりとなることが期待できる。
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