2022 Fiscal Year Final Research Report
Elucidation of cytotoxicity mechanism for oncolytic virus by genome-editing high-throughput knockout
| Project/Area Number |
19K16500
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 48030:Pharmacology-related
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| Research Institution | Jikei University School of Medicine (2022)
The University of Tokyo (2019, 2021) |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | ウイルス療法 |
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| Outline of Final Research Achievements |
In this study, we developed cancer cell line populations in which various genes were deleted by genome editing technology and identified genes required for cytotoxicity of an oncolytic virus, coxsackievirus group B type 3 (CVB3), using them. used the cancer cell populations to generate coxsackievirus group B type 3, an oncolytic virus.
By understanding the cytotoxicity mechanism of CVB3 in detail, it becomes possible to scientifically set adaptation criteria. In this time, we analyzed the results of next-generation sequencing, identified the barcode sequence integrated into the lentivirus, and successfully identified various gene candidates of CVB3 cytotoxicity.
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| Free Research Field |
ウイルス療法
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、これまでにCVB3の細胞傷害性に関して報告のない遺伝子が多く見つかっており、さらに報告のないシグナル経路がウイルス増殖に関連している可能性が示唆され、今後のウイルス療法開発に重要な鍵となることが予想される。
また、本研究で同定された遺伝子が他のエンテロウイルスに関与しているかどうか解析することにより、他のエンテロウイルスに対する治療薬の開発に繋がる可能性がある。
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