2020 Fiscal Year Annual Research Report
The role of O-GlcNAcylation on Notch1 in the self-renewal of muscle satellite cells
Project/Area Number |
19K16517
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Research Institution | Nagoya University |
Principal Investigator |
LO PEIWEN 名古屋大学, 医学系研究科, 研究機関研究員 (20822406)
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Project Period (FY) |
2019-04-01 – 2021-03-31
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Keywords | O-GlcNAcylation / Notch1 / aging / muscle |
Outline of Annual Research Achievements |
We speculated declined muscle self-renewal with aging is mediated partially by the O-GlcNAcylation on Notch1. To understand if the O-GlcNAcylation status of Notch1 is altered with aging, I established the inducible FLAG-Notch1 transgenic mice. Afterwards, O-GlcNAcylation on Notch1 protein extracted from aged and younger mice will be applied in glycosylation analysis. Albeit Eogt didn’t be detected in satellite cells of muscle, I found Eogt distributes only in “certain” myofibers. In further experiments, I found Eogt highly overlaps myofiber type2A. The mouse hanging assay also shows Eogt knockdown reduces muscle contractibility. It suggests that Eogt is involved in muscle contractibility by mediating myofiber types. This study facilitates to ameliorate mobility of aged people.
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