Development of control technology for metastatic ability and invasion activity in human cancer cells by epigenome editing

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16521
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Hosoba Kosuke 広島大学, 統合生命科学研究科(理), 助教 (20781264)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: エピゲノム編集 / CDH1遺伝子 / 固形癌

Outline of Final Research Achievements

The result of wound healing assay revealed that migration activities of TREE system-induced cancer cells ware reduced. Next, cell invasion assay was performed. The rate of cells that enter the micro pores was decreased in TREE system-induced cells. These result suggested that increase of CDH1 gene expression by TREE system suppressed metastatic ability and migration activity of human cancer cells.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究によりエピゲノム編集法の固形癌由来細胞株における効果を実証出来た。所属研究室で開発された新規エピゲノム編集ツールを用いてCDH1遺伝子の発現量をコントロールすることで癌細胞の浸潤能及び転移能が抑制出来ることが示唆された。本研究で得られた成果は基礎生物学的な領域だけでなく、今後の抗癌剤開発などを含む癌治療戦略に新たな知見を与える可能性がある。