2019 Fiscal Year Research-status Report
Studies on the association of lipid hydroperoxides and Alzheimer's disease by LC/MS
| Project/Area Number |
19K16531
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| Research Institution | Hokkaido University |
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Principal Investigator |
陳 震 北海道大学, 保健科学研究院, 助教 (60802634)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | oxdation stress / oxidized lipids / lipid hydroperoxides / lipidomics / plasmalogen / antioxidant / LC/MS |
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| Outline of Annual Research Achievements |
We have developed the evaluation system of both biology and chemistry. By using the human hepatocyte as a pilot study, we tested oxidation stress model and its potential protection trial.
We chose the heavy metal lead (Pb) stimulation as the oxidation model, and used the bioactive phospholipid plasmalogen as the antioxidant. The present work revealed that Pb-mediated oxidative stress reduced the HepG2 cell viability in association with the generation of ROS, the hydroperoxides as the oxidized phospholipids, and the lysophospholipids as the degradation products. These adverse effects of Pb were largely eliminated by plasmalogen species with different headgroups and sn-2 fatty acyls via both a sacrificial trap for ROS and a mechanistic activation of Nrf-2-dependent antioxidant enzymes.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The evaluation system, including the cell culture, the ROS and antioxidant enzyme assay, the lipidomic tests of cells, have been established.
The nerve cells (PC12) have been purchased and cultured.
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| Strategy for Future Research Activity |
We will apply the current workflow onto the PC12 cells.
Because nerve cells are different from liver cells, in the following experiment we will notice any new findings and specific characteristics.
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