2020 Fiscal Year Annual Research Report
Studies on the association of lipid hydroperoxides and Alzheimer's disease by LC/MS
| Project/Area Number |
19K16531
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| Research Institution | Hokkaido University |
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Principal Investigator |
陳 震 北海道大学, 保健科学研究院, 講師 (60802634)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | oxdation stress / oxidized lipids / lipid hydroperoxides / lipidomics / plasmalogen / antioxidant / LC/MS / PC12 cells |
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| Outline of Annual Research Achievements |
Alzheimer’s disease (AD) has become a serious threaten worldwide, and oxidative stress are considered play a critical role in its pathological development. In this project, the in-vitro model by PC12 cells was applied to study the association between oxidative stress in AD and lipids, especially the oxidized lipids.
During the past year, we established the in-vitro model by differentiating PC12 cells and exposing to H2O2, and then assessed the cell morphology, viability, and reactive oxygen species level. Then, the lipids of cells were extracted and analyzed by the optimized LC/MS method. As a result, the oxidized lipids, such as the hydroperoxides of triglycerides (TG-OOH), phosphatidylcholine (PC-OOH), and phosphatidylethanolamine (PE-OOH), accumulated along with the increased concentration of H2O2, suggesting the oxidation-induced damage to intact lipids. Moreover, in terms of the functional lipids, including plasmalogen ethanolamine (PlsEtn) and cardiolipin (CL), their amounts decreased with a H2O2 dose-dependent manner. These results indicated the disorder of lipid metabolism occurred in the current in-vitro AD model.
Besides, since the functional phospholipid plasmalogen was supposed to be an anti-oxidative stress component and was proved in the pilot study, the investigation of plasmalogens with regard to food chemistry and food science were also performed.
This work not only showed the possibility that lipid hydroperoxides positively correlated to AD, but also proposed the functional phospholipid plasmalogen as an anti-lipid hydroperoxidation component from diet.
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Research Products
(4 results)
