Analysis of molecular mechanisms involved in fibrosis and inflammation by comparing subtypes of well-differentiated liposarcoma

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16563
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Yokohama City University
• Principal Investigator: KATO Ikuma 横浜市立大学, 医学部, 助教 (80644939)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 高分化型脂肪肉腫 / 組織像 / 遺伝子発現

Outline of Final Research Achievements

While well-differentiated liposarcoma commonly has MDM2 gene amplification, they exhibit a heterogeneous histological appearance. To investigate molecular mechanisms involved in fibrosis and inflammation in addition to MDM2 gene amplification, we analyzed RNA sequencing data of 6 case having both lipoma-like and sclerosing areas. As a result, 9 highly expressed RNAs in sclerosing areas were found, some of which could be studied for protein expression. However, no specificity for the subtype was proven after studying many cases.

Free Research Field

人体病理学

Academic Significance and Societal Importance of the Research Achievements

比較的均一な遺伝子異常を有するとされる軟部腫瘍において、組織学的不均一性を説明する分子機構の解明を試みたことは、より複雑な遺伝子異常を有する癌腫における組織学的不均一性の解析にも寄与すると考えられた。また、一般的な施設で保管されている、ホルマリン固定パラフィン包埋材料からも一定の質の網羅的RNA発現データを得られたことは、今後に類似の手法で解析を試みる際の足掛かりとなった。