Functional analysis of proteins involved in stepwise progression found by proteomic analysis of small lung adenocarcinoma

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K16578
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: University of Tsukuba
• Principal Investigator: Dai Tomoko 筑波大学, 附属病院, 研究員 (20835194)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 肺腺癌 / プロテオミクス

Outline of Final Research Achievements

We performed quantitative proteomics analysis of adenocarcinoma in situ (AIS) and small invasive lung adenocarcinoma which harbored EGFR mutation. Among the proteins those showed higher expression in early invasive lung adenocarcinoma than AIS by proteomics analysis, we could confirm six proteins (CRABP2, NDRG1, DHCR24, AK4, PIP4K2C, IFITM3) those are significantly higher in small invasive adenocarcinoma than AIS by western blot analysis. Using lung adenocarcinoma tissue microarrays, immunohistochemistry was performed, and five proteins (CRABP2, NDRG1, DHCR24, AK4, IFITM3) were significantly correlated with poor prognosis.

Free Research Field

肺腺癌

Academic Significance and Societal Importance of the Research Achievements

肺腺癌のドライバー遺伝子であるEGFR変異は前癌病変や予後のよい上皮内腺癌においても検出され、肺腺癌の悪性化にはEGFR変異以外の因子も関わっている可能性がある。小型肺腺癌のプロテオミクス解析からAISより小型浸潤性肺腺癌で発現が高く、肺腺癌多数症例で予後との相関がみられた5つのタンパク（CRABP2, NDRG1, DHCR24, AK4, IFITM3）が見出された。これらの5つのタンパクは、肺腺癌の悪性度に早期から関与する可能性がある。