Classification and molecular epidemiological analysis of carbapenem resistant strains including P. asiensis and its related species.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K16652
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49050:Bacteriology-related
• Research Institution: Juntendo University
• Principal Investigator: Tohya Mari 順天堂大学, 医学部, 助教 (20804694)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: Pseudomonas / 新菌種 / 全ゲノム解析 / 薬剤耐性

Outline of Final Research Achievements

Carbapenem-resistant isolates obtained from clinical samples in Japan and Myanmar were identified as P. asiatia (revised from P. asiensis) and P. juntendi, which are novel species belonging to the P. putida group, and P. yangonensis, which is a novel species belonging to the P. oleovorans group. The drug-resistance associated genes encoding carbapenemases were detected from whole genome sequencing data of these novel species strains. The phylogenetic anajysis also conducted and reported with the above results.
Forty-two clinical isolates, identified as P. putida or P. fluorescens in clinical laboratories, were re-identified using whole genome sequencing data of them. Nine novel species were proposed from the 42 isolates data.

Free Research Field

細菌学

Academic Significance and Societal Importance of the Research Achievements

研究代表者はPseudomonas aeruginosa（緑膿菌）以外のPseudomonas属菌は臨床検査室では誤同定されやすいことを明らかにした。全ゲノムデータをもとに、カルバペネム耐性株から3菌種、日本の医療施設から分離された菌株から9菌種の新たなPseudomonas属菌を提唱した。また臨床分離株はほとんどが誤同定されていた結果から、過去の分離株についても見直す必要性を提唱している。さらにPseudomonas属の既存の同定システムの改良やデータベースの更新が必要なことも示唆している。