Surveillance of clinical isolates including antimicrobial resistant genes derived from livestock

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K16657
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49050:Bacteriology-related
• Research Institution: Toin University of Yokohama
• Principal Investigator: HASUNUMA Yuya 桐蔭横浜大学, 医用工学部, 講師 (70643013)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: ESBL産生菌 / floR / qnr / AMR / β-ラクタマーゼ

Outline of Final Research Achievements

Antimicrobial resistant bacteria is of global concern. In particular, the drug-resistant bacteria often include antimicrobial resistant genes, that are thought to origin from livestock and environment, among clinical isolates. In this study, we focused on floR gene, which is a resistance gene to florphenicol used in livestock. The prevalent rates of floR were 1.5%, 6.4%, and 2.9% in ESBL producing Escherichia coli from clinical isolates, retail meats and healthy humans, respectively. Furthermore, the plasmid possessed the isolates from retail meat had a potential of high frequency of transconjugation.
In addition, the prevalent rate of the qnr gene, which was carried with floR in the strain, was investigated. The prevalence of qnr gene in non-ESBL and ESBL-producing E. coli was 2% and 4%, respectively, and qnrS were determined among all strain.

Free Research Field

薬剤耐性菌

Academic Significance and Societal Importance of the Research Achievements

本研究において、家畜からヒトへ薬剤耐性菌が拡散したことを示唆する耐性遺伝子マーカーとしてfloR遺伝子を提案することができたと考える。この成果は、ヒト環境における薬剤耐性遺伝子流入経路の一つを明らかにすることにつながり、新たな薬剤耐性菌拡散の分子生物学的背景をモニタリングできる可能性がある。このことは、薬剤耐性菌の拡散を制御するための新たな方法へと繋がると考えられ、さらには、将来的に薬剤耐性菌による感染症の減少、患者入院期間の短縮、医療費の削減にも貢献する。