2019 Fiscal Year Research-status Report
SMAD-STAT immune signaling network in lung cancer microenvironment
Project/Area Number |
19K16699
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Research Institution | Tokyo Medical University |
Principal Investigator |
尹 晶煥 東京医科大学, 医学部, 兼任助教 (30748885)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | Lung cancer / Anti-tumor immunity / Dendritic cell / T cell / TGF-beta / SMADs / STATs |
Outline of Annual Research Achievements |
<Plan 1: Roles of SMADs in DCs and T cells in lung mucosal immune homeostasis> T cell and DC-specific Smad2 and Smad4 deficient (KO) mice showed normal immune phenotypes. Smad3 KO mice showed deficiency in a DC subset at the steady-state condition. <Plan 2: Roles of SMADs in DCs and T cells in lung cancer> I have established Lewis lung carcinoma cell line engineered to express GFP or the firefly luciferase gene (luc2) for orthotopic lung cancer model. <Plan 3: Effects of SMAD deficiency on STAT signaling in DCs and T cells in lung cancer> I have found that increased conventional DCs (cDCs) by SMAD3 deficiency showed significantly enhanced phosphorylation of STAT3. <Plan 4: Mechanisms how SMAD-STAT signaling networks regulate DCs and T cells in anti-lung cancer immunity> I have generated the various constitutively active or inactive mutants of SMADs or STAT3 using adeno-associated virus system.
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Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
As stated in the summary, I have made a progress in accordance with the proposed plans.
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Strategy for Future Research Activity |
I will continue the research as proposed. I will finish revision of the submitted manuscript to publish the results on the role of SMAD3 on DC development (cf. Plan 1).
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Causes of Carryover |
Due to the pandemic, I could not spend all the fiscal budget. I will spend the balance for purchasing the antibodies and reagents required for the orthotopic Lewis lung carcinoma model and mechanistic studies this year.
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Research Products
(1 results)
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[Presentation] SMAD3 Determines Conventional versus Plasmacytoid Dendritic Cell Fates2019
Author(s)
Jeong-Hwan Yoon, Eunjin Bae, Jin Soo Han, Seok Hee Park, Susumu Nakae, InKyu Lee, Ji Hyeon Ju, Isao Matsumoto, Takayuki Sumida and Mizuko Mamura
Organizer
The 48th Annual Meeting of The Japanese Society for Immunology, Hamamatsu, Japan
Int'l Joint Research