Elucidation of inflammatory cytokines via macrophage in the tumor microenvironment of Ewing's sarcoma

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K16717
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 50010:Tumor biology-related
• Research Institution: Kyushu University
• Principal Investigator: Iida Keiichiro 九州大学, 大学病院, 助教 (70782621)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: マクロファージ / がん微小環境 / 肉腫

Outline of Final Research Achievements

Recent studies have highlighted the importance of cells from the tumor stroma. Among them, tumor-associated macrophages (TAMs) have an important role in solid-tumor behavior including invasion, angiogenesis, and metastasis. We have reported the elevated C-reactive protein concentration and white blood cell counts were significantly associated with higher infiltration of TAMs, and TAMs enhanced the progression of Ewing sarcomas through angiogenesis. These results indicated the importance of the regulation factor of macrophage. MCP-1(Monocyte Chemotactic Protein-1) is one of the inflammation cytokines and the most potent chemoattractant for monocytes which belong to the C-C chemokine group. Our study reported the MCP-1 from Ewing tumor cells activated macrophages and the matrix metalloproteinase secreted by the activated macrophage produced angiogenesis and tumor progression.

Free Research Field

肉腫 がん微小環境

Academic Significance and Societal Importance of the Research Achievements

炎症性サイトカイン安定発現細胞株を作成し、腫瘍に浸潤したマクロファージを回収、解析することにより、肉腫とマクロファージの相互関係について解析を行った。Ewing肉腫では有効な化学療法は限られている。本研究の結果により、Ewing肉腫のがん微小環境を標的とした分子標的治療の可能性を開くことが期待される。