Role of kinesin/dynein adaptor JSAP in reactive oxygen species-induced cell death and lysosome positioning

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16737
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 50010:Tumor biology-related
• Research Institution: Kanazawa University
• Principal Investigator: I.Ketut Gunarta 金沢大学, がん進展制御研究所, 助教 (90838393)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: JSAP / JLP / JSAP1 / Curcumin / Autophagy / Lysosome

Outline of Final Research Achievements

The ROS level is tightly regulated by a cell as it can oxidize molecules inside a cell and ultimately lead to cell death. ROS inducing agent, such as curcumin, has been reported to alter lysosome localization in a cancer cell. We found that JSAP, a family of motor protein adaptors, is involved in lysosome transport in response to curcumin. We also clarified the relationship between JSAP-mediated lysosome transport and curcumin-induced cell death in cancer. Our study suggested that the closely related member of the JSAP family, JSAP1 and JSAP2 (also known as JLP), play a distinct function in curcumin-induced stress.

Free Research Field

腫瘍生物学関連

Academic Significance and Societal Importance of the Research Achievements

ROSレベルを上昇させ、細胞死を誘発する治療薬は、代替の癌治療を提供します。 ウコン（Curcuma longa）に由来する化合物であるクルクミンは、インビトロおよびインビボで細胞死を誘発する有望な効果があるため、魅力的な薬剤候補です。 この研究の結果は、細胞内輸送が、新しい治療戦略の開発につながる可能性のある急性酸化ストレスの生存にどのように関連していたかについてのより良い理解を与えることが期待されます。